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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR)

Anti -TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant E B IP IH IC FC
The Trk proto-oncogene encodes a 140kDa, membrane- spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10e-9M. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).
Catalog #T8663-09
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:2500-1:5000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Flow Cytometry: 1:30
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E601
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues surrounding tyrosine 791 of human Trk A.
SpecificityRecognizes both phosphorylated and unphosphorylated human Trk A. Species crossrectivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesNeurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, p140-TrkA, Trk-A


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