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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -TrkB (Tyrosine Kinase Receptor B)

Anti -TrkB (Tyrosine Kinase Receptor B)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The Trk (tropomyosin-receptor-kinase) proto-oncogene family of receptor tyrosine kinases contains four members, TrkA, TrkB, TrkC and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkB is the high-affinity to the binding brain-derived neurotrophic factor (BDNF) receptor and neurotrophin-4/5 (NT-4/5). TrkB is autophosphorylated on five cytoplasmic tyrosines. The binding of BDNF to TrkB receptor causes many intercellular cascades to be activated such as promoting survival of sensory neurons mainly through Shc site-independent pathways. The signaling activity of these receptors includes the Ras-MAP kinase pathway and the PI3 Kinase pathway. These receptors are responsible for resistance to apoptosis, obesity, Alzheimer’s disease and mood disorders.
Catalog #T8663-20
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5-2ug/ml detected TrkB in mouse brain membrane protein preparations.
Optimal dilutions to be determined by the researcher.
Recommended ControlBrain tissue
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenThe entire extracellular domain (corresponding to residues 1-429) of the rat TrkB receptor, expressed in COS cells (NP_036863).
Species Sequence HomologyMouse; 97%, human; 88%
SpecificityRecognizes rat TrkB, Mr 145kD, as well as an additional protein, Mr ~90kD, which is likely a TrkB degradation product.
Species CrossreactivityMouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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