Troponin I, Cardiac, Human (cTnI, Tn-I, hTn-I)
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| Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character. | | | Catalog # | T8665-13 | | Tissue-specific Troponin Subtypes | Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) | | Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) | | Cardiac troponin I, TNNI3 (19q13.4, 191044) | | Applications | Suitable for use in ELISA. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | 77108-40-8 | | Source | Human heart tissue | | Purity | 95% by SDS-PAGE | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 8M Urea, 0.05M Tris HCl, 0.015M beta-mercaptoethanol, 2mM EDTA, pH 7.4. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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