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Troponin I, Cardiac, Bovine (Troponin I, Cardiac Muscle, Cardiac Troponin I, TNNI3)
Troponin I, Cardiac, Bovine (Troponin I, Cardiac Muscle, Cardiac Troponin I, TNNI3)
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| Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character. | | | Catalog # | T8665-13Q | | Source | Bovine cardiac tissue. | | Molecular Weight | ~24kD | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~24kD | | Source | Bovine cardiac tissue | | Purity | ~90% (SDS-PAGE). Purified by Ion Exchange chromatography and calcium dependent affinity chromatography. | | Concentration | As reported | | Form | Supplied as a liquid in 8M urea, 50mM tris-HCl,100mM sodium chloride, 2mM EDTA, pH7.4, 5mM beta-mercaptoethanol. Preservative free. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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