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You are here:Home » Molecular Biology » MB-Cardiac Markers » Troponin I, Cardiac, Porcine (Troponin I, Cardiac Muscle, Cardiac Troponin I, TNNI3)

Troponin I, Cardiac, Porcine (Troponin I, Cardiac Muscle, Cardiac Troponin I, TNNI3)

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Specifications

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter I is given due to its inhibitory character.
Catalog #T8665-13S
SourcePorcine cardiac tissue.
Molecular Weight~24kD
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~24kD
SourcePorcine cardiac tissue
Purity~95% (SDS-PAGE). Purified by Ion Exchange chromatography and calcium dependent affinity chromatography.
ConcentrationAs reported
FormSupplied as a liquid in 8M urea, 50mM tris-HCl, 1mM EDTA, pH7.4, 15mM beta-mercaptoethanol. Preservative free.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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