Anti -Troponin I, Cardiac, Human (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7,
MGC116817, Troponin-I, Troponin I Cardiac Muscle, TNNC 1, TNNC1, Troponin I Type 3
Cardiac, TNNI3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
|
E B IP |
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| Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character. | | | Catalog # | T8665-14E | | The tissue specific subtypes are | Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) | | Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) | | Cardiac troponin I, TNNI3 (19q13.4, 191044) | | Applications | Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blotting: 1:1000-1:2000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 9F117 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS, pH 7.2. | | Purity | As reported | | Immunogen | Full length protein (Human). Epitope: 87 - LGFAE - 91. | | Specificity | Reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Does not crossreact with skeletal muscle troponin I. Species Crossreactivity: Crossreacts with Human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Troponin I, Cardiac, Human (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, Troponin-I, Troponin I Cardiac Muscle, TNNC 1, TNNC1, Troponin I Type 3 Cardiac, TNNI3) |
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