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You are here:Home » Antibodies » Abs to Cardiac Markers » Anti -Troponin I, Cardiac, Human (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, Troponin-I, Troponin I Cardiac Muscle, TNNC 1, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

Anti -Troponin I, Cardiac, Human (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7,
MGC116817, Troponin-I, Troponin I Cardiac Muscle, TNNC 1, TNNC1, Troponin I Type 3
Cardiac, TNNI3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse E B IP
In clinical studies of new drugs, new methods of surgery are carried out on experimental animals. The effects of the new technologies on cardiac function and on cardiac myocyte viability can be important. The majority of the anti-cardiac Troponin I MAbs have wide specificity and recognize not only human cardiac Troponin I but also cardiac Troponin I from other species. Several combinations of MAbs were tested and could be used for quantitative detection of cardiac Troponin I in blood of different species. An assay utilizing T8665-14M as a capture and T8665-15E as detection gives equal response with antigens from different animal species. Other MAb pairs, with epitopes located in different parts of troponin molecule, could also be used for the same purpose.
Catalog #T8665-14M
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: Predicted molecular weight 24kD.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9F123
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2.
PurityAs reported
ImmunogenFree human cardiac troponin and/or native troponin complex. Epitope: 27-43 aa
SpecificityThis antibody is reacting with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (In the presence of 5 mM EDTA). It is not affected by heparin, phosphorylation, oxidation and troponin complex formation. This antibody does not crossreact with skeletal muscle troponin I. Species Crossreactivity: Crossreacts with Mouse, Rat and canine. Expected to crossreact with Human due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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