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You are here:Home » Antibodies » Abs to Cardiac Markers » Anti -Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

Anti -Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817,
TNNC1, Troponin I Type 3 Cardiac, TNNI3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Cardiac Troponin T, a 36kD protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms.
Catalog #T8665-15
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter I is given due to its inhibitory character.
Tissue Specific SubtypesSlow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042)
Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043)
Cardiac troponin I, TNNI3 (19q13.4, 191044)
ApplicationsSuitable for use in ELISA, Western Blotting and Immunoaffinity Purification. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
HybridomaHybridization of Sp 2/0 myeloma cells with spleen cells from Balb/c mice.
SourceAscites
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No1.BB.135
HostMouse
SourceHuman
Concentration~3mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity>90%. Purified by chromatography on protein A sepharose. Purity is tested by electrophoresis.
ImmunogenFree human cardiac troponin I.
SpecificityReacts with free cardiac Troponon I (cTnl) and cTnl forming complexes with other troponin components. Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Recognizes an epitope located between amino acid residues 186 and 192 of cTnI. Crossreactivity with skeletal troponin I from human, rat and mouse is observed.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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