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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Catalog #	T8665-15C
Applications	Suitable for use in Western Blot, Troponin I Immunoassay, Troponin I immunoaffinity purification and Immunochromatographic applications. Other applications not tested.
Recommended Dilution	Optimal dilution determined by the researcher.
Hybridoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Recommended Pair	Suitable for use as the detection or conjugate (label) antibody.
	Matched to T8665-19B as the capture or solid phase antibody.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	1.BB.138
Host	Mouse
Source	Human
Concentration	~10mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	>90%. Protein A Sepharose Chromatography. Purity is tested by electrophoresis.
Immunogen	Native troponin complex.
Specificity	Reacts equally with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. Recognizes an epitope located between amino acid residues 25 and 40. No crossreactivity with skeletal muscle troponin I.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.