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Anti -Troponin I, Cardiac (cTnI) (Biotin)
Anti -Troponin I, Cardiac (cTnI) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character. | | | Catalog # | T8665-16E1 | | Applications | Suitable for use in Western Blot and sandwich ELISA. Other applications not tested. | | Recommended Dilutions | Optimal dilutions to be determined by the researcher. | | Hybridoma | Sp2/0 myeloma cells with spleen cells of Balb/c mice. | | Recommended Pairs | Detection: | | T8665-19B: Troponin I, Cardiac, aa41-49 | | T8665-16R: Troponin I, Cardiac, aa169-178 | | Capture: | | T8665-16E1: Troponin I, Cardiac | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 7E143 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS. No preservative added. Labeled with Biotin. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Human troponin complex | | Specificity | Recognizes free human cardiac troponin I (cTnl) and cTnl forming complexes with other troponin components. Not effected by heparin, phosphorylation and oxidation. Recognizes an epitope located between amino acid residues 83-93 of cTnI. No crossreactivity with skeletal muscle troponin I. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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