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You are here:Home » Antibodies » Abs to Cardiac Markers » Anti -Troponin I, Cardiac (cTnI)

Anti -Troponin I, Cardiac (cTnI)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #T8665-16N
ApplicationsSuitable for use in Western Blot, ELISA and immunoaffinity purification. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7E144
HostMouse
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenNative troponin complex
SpecificityReacts equally with free cardiac Troponin I (cTnI) and cTnI forming complexes with other troponin components (In the presence of 5mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. Recognizes an epitope located between aa130-145 of cTnI. Crossreactivity with skeletal troponin I is 5-7%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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