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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B

Catalog #	T8665-16P
Applications	Suitable for use in Troponin I immunoassay, troponin I immunoaffinity purification and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1-2ug
	Optimal dilutions to be determined by the researcher.
	Storage and Stability:
	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	7E140
Host	Mouse
Source	Human
Concentration	~3mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Native troponin complex
Specificity	Reacts equally with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Recognizes an epitope located between amino acid residues 15 and 25 of cTnI. No cross-reactivity with skeletal muscle troponin I.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.