| Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character. |
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| Catalog # | T8665-16R |
| The tissue specific subtypes are | Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) |
| Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) |
| Cardiac troponin I, TNNI3 (19q13.4, 191044) |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Recommended Pair | Suitable for use as the detection or conjugate (label) antibody. |
| Matched to T8665-16E as the capture or solid phase antibody. |
| Also suitable as the detection or conjugate (label) antibody with T8665-19B. Matched to T8665-16E and T8665-16H as the capture or solid phase antibodies. |
| Hybridoma | Hybridization of Sp 2/0 myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgM |
| Clone No | 7E148 |
| Host | Mouse |
| Source | Human |
| Concentration | ~4mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Free human Cardiac Troponin I |
| Specificity | Recognizes an epitope located between aa169-178 of cTnI. Reacts equally with free cardiac troponin I (cTnl) and cTnI forming complexes with other troponin components (in the presence of 5mM EDTA). Not affected by heparin, phosphorylation and oxidation. No crossreactivity with skeletal muscle troponin I. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
