Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Chicken Purified E B IH

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place Because of which myosin can not bind the actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confirmational changes which leads to dislocation of troponin I and finally tropomyosin leaves the the binding site for myosin on actin leading to contraction of muscle. The letter I is given due to its inhibitory character.
Catalog #	T8665-18
Applications	Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended Dilution	ELISA: Use as a capture.
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies	I1906 IgY, Chicken Pab Rb x Ch
	I1906-10 IgY, Chicken-HRP Rb x Ch
	I1906-12 IgY, Chicken-FITC Rb x Ch
	I1906-14 IgY, Chicken-AP Rb x Ch
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Polyclonal
Isotype	IgY
Host	Chicken
Source	Human
Concentration	~5mg/ml
Form	Supplied as a liquid in TBS, pH 7.4, 0.1% sodium azide.
Purity	Purified
Immunogen	Human Troponin I (Cardiac)
Specificity	Recognizes human Troponin I (Cardiac).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.