Anti -Troponin I, Cardiac, aa23-29 (cTnI)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character. | | | Catalog # | T8665-18B | | The tissue specific subtypes are | Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) | | Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) | | Cardiac troponin I, TNNI3 (19q13.4, 191044) | | Applications | Suitable for use in ELISA, Western Blot and Immunoaffinity Purification. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Hybridoma | SP2/0 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 1.BB.143 | | Host | Mouse | | Source | Human | | Concentration | ~10mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. | | Purity | Purified by Protein A chromatography. | | Immunogen | Native troponin complex. Epitope: aa 23- 29. | | Specificity | Reacts equally with free cardiac troponin I (cTnI) and cTnl forming complexes with other troponin components. Not affected by heparin, phosphorylation, and oxidation. Species Crossreactivity: Human. bovine, porcine, goat, canine, rabbit, feline, rat and mouse. Does not crossreact with cTnI from fish or skeletal muscle troponin I. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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