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You are here:Home » Antibodies » Abs to Cardiac Markers » Anti -Troponin I, cardiac muscle, DEPHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1)

Anti -Troponin I, cardiac muscle, DEPHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Troponin I is a 24kD subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death.
Catalog #T8665-18C
Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No10F562
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenDephosphorylated form of human cardiac Troponin I.
SpecificityRecognizes the dephosphorylated form of human cardiac Troponin I (cTnI). It specifically recognizes aa20-24 on cTnI. The antibody will recognise free dephosphorylated cTnI or dephosphorylated cTnI in complex with other troponin components. Does not cross react with phosphorylated cTnI or with skeletal TnI. Species Crossreactivity: pig (weakly).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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