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Anti -Troponin I, cardiac muscle, DEPHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1)
Anti -Troponin I, cardiac muscle, DEPHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| Troponin I is a 24kD subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death. | | | Catalog # | T8665-18C | | Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts. | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Hybridoma | Sp2/0 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 10F562 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Dephosphorylated form of human cardiac Troponin I. | | Specificity | Recognizes the dephosphorylated form of human cardiac Troponin I (cTnI). It specifically recognizes aa20-24 on cTnI. The antibody will recognise free dephosphorylated cTnI or dephosphorylated cTnI in complex with other troponin components. Does not cross react with phosphorylated cTnI or with skeletal TnI. Species Crossreactivity: pig (weakly). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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