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You are here:Home » Molecular Biology » MB-Cardiac Markers » Troponin I, Cardiac, Recombinant, Human (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

Troponin I, Cardiac, Recombinant, Human (cTnI, CMH7, Familial Hypertrophic
Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

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Specifications

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter I is given due to its inhibitory character.
Catalog #T8665-18G
Recombinant Human cTnI is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 24,016D.
Tissue-specific Troponin SubtypesSlow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042)
Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043)
Cardiac troponin I, TNNI3 (19q13.4, 191044)
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Protein ContentProtein quantitation was carried out by:
0.25-5.0mg/ml Bradford assay vs. BSA.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, add 0.1% HSA or BSA, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight24kD
SourceRecombinant, Human (E. coli).
Purity95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin:< 0.1ng/ug (IEU/ug).
Concentration~1mg/ml
FormSupplied as a liquid in 6M Urea, 50mM Tris, pH 8.0.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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