Troponin I, Cardiac, Recombinant, Human (cTnI, Tn-I, hTn-I)
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| Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character. | | | Catalog # | T8665-18G | | Recombinant Human cTnI is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 24,016D. | | Tissue-specific Troponin Subtypes | Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) | | Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) | | Cardiac troponin I, TNNI3 (19q13.4, 191044) | | Dimers and Aggregates | 1% as determined by silver-stained SDS-PAGE gel analysis. | | Protein Content | Protein quantitation was carried out by: | | 0.25-5.0mg/ml Bradford assay vs. BSA. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, add 0.1% HSA or BSA, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 24kD | | Source | Recombinant, Human (E. coli). | | Purity | 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin:< 0.1ng/ug (IEU/ug). | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 6M Urea, 50mM Tris, pH 8.0. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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