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You are here:Home » Antibodies » Abs to Cardiac Markers » Anti -Troponin I, Cardiac, phosphorylated (Ser23/24) (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

Anti -Troponin I, Cardiac, phosphorylated (Ser23/24) (cTnI, CMH7, Familial Hypertrophic
Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter I is given due to its inhibitory character.
Catalog #T8665-18P
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser23/24 of human troponin I (cardiac) (KLH).
SpecificityRecognizes endogenous levels of human cardiac troponin I only when phosphorylated at serine 23/24 at 28kD. Species Crossreactivity: mouse, rat. Does not crossreact with phosphorylated skeletal muscle troponin I.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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