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You are here:Home » Antibodies » Abs to Cardiac Markers » Anti -Troponin I, Cardiac, aa87-91 (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

Anti -Troponin I, Cardiac, aa87-91 (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7,
MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter I is given due to its inhibitory character.
Catalog #T8665-19D
The tissue specific subtypes areSlow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042)
Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043)
Cardiac troponin I, TNNI3 (19q13.4, 191044)
ApplicationsSuitable for use in ELISA, Western Blot and Immunoaffinity Purification. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Recommended PairSuitable for use as the capture or solid phase antibody.
Matched to T8665-19B as the detection or conjugate (label) antibody.
Recommended PairSuitable for use as the detection or conjugate (label) antibody.
Matched to T8665-19A or T8665-19B as the capture or solid phase antibody.
HybridomaSP2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.BB.146
HostMouse
SourceHuman
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity>90% Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.
ImmunogenFree human cardiac troponin I.
SpecificityRecognizes free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. Recognizes an epitope located between amino acid residues 87 and 91 of cTnI. No crossreactivity with skeletal muscle troponin I. Species Crossreactivity: Human, bovine, porcine, goat, canine, rabbit and feline. Not reactive with cTnI from rat, mouse or fish.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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