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You are here:Home » Antibodies » Abs to Cardiac Markers » Anti -Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

Anti -Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817,
TNNC1, Troponin I Type 3 Cardiac, TNNI3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter I is given due to its inhibitory character.
Catalog #T8665-20A
Tissue-specific Troponin SubtypesSlow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042)
Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043)
Cardiac troponin I, TNNI3 (19q13.4, 191044)
ApplicationsSuitable for use in ELISA., Immunoaffinity Purification, and Western Blot.
Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Matched PairSuitable for use as the capture or solid phase antibody.
Matched to T8665-20B as the detection or conjugate (label) antibody.
HybridomaClones have been derived from hybridization of Sp2/0myeloma cells with spleen cells of Balb/c mice immunized with free human cardiac troponin and/or native troponin complex.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.BB.147
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity>90%. Purified by Protein A affinity chromatography.
ImmunogenNative troponin complex.
SpecificityReacts equally with free cardiac troponin I (cTnI) and with cTnI forming complexes with other troponin components (in the presence of 5mM EDTA). Recognizes an epitope located between amino acid residues 86 and 90 of cTnI. Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Has no crossreaction with skeletal muscle troponin I.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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