| Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character. |
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| Catalog # | T8665-20C |
| The tissue specific subtypes are | Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) |
| Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) |
| Cardiac troponin I, TNNI3 (19q13.4, 191044) |
| Applications | Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Recommended Pairs | Detection: |
| T8665-20C: Troponin I, Cardiac, aa27-39 (cTnI) |
| Capture: |
| T8665-16A: Troponin I, Cardiac (cTnI) |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Highly purified human cardiac Troponin I |
| Specificity | Recognizes human cardiac Troponin I. Crossreactivity with human skeletal Troponin I is 0.02% by ELISA. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
