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Specifications

Three troponin I genes have been identified in vertebrates that encode the isoforms expressed in adult cardiac muscle (TNNI3), slow skeletal muscle (TNNI1) and fast skeletal muscle (TNNI2), respectively. Skeletal isoforms of Troponin I were suggested to be used as markers of acute and chronic skeletal muscle injuries.
Catalog #	T8665-21L
Source	Sheep skeletal muscle.
Molecular Weight	~22kD
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight	~22kD
Source	Sheep skeletal muscle
Purity	~95% (SDS-PAGE). Purified by Ion Exchange chromatography and calcium dependent affinity chromatography.
Concentration	As reported
Form	Supplied as a liquid in 8M urea, 50mM tris-HCl, 100mM sodium chloride, 1mM EDTA, pH7.4,
15mM beta-mercaptoethanol. Preservative free.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.