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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure.
Catalog #	T8665-22A
Applications	Suitable for use in ELISA, Western Blot and Immunoaffinity Purification. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Recommended Pair	Suitable for use as the capture or solid phase antibody. Matched to T8665-22B as the detection or conjugate (label) antibody.
	Also suitable for use as the detection or conjugate (label) antibody. Matched to T8665-22C as the capture or solid phase antibody.
Hyrbidoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	1.BB.154
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Free human Cardiac Troponin T.
Specificity	Recognizes human Cardiac Troponin T. Recognizes an epitope located betweeen aa95-181. Species Crossreactivity: mouse, rat and canine. Does not crossreact with human skeletal Troponin T.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.