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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IF

Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure.
Catalog #	T8665-23A
Application	Suitable for use in Electron Microscopy, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
	Optimal dilutions to be determined by the researcher.
Positive Control	Cardiac muscle
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.590
Host	Mouse
Source	Rabbit
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T8665-23AX.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Purified rabbit cardiac troponin T isoform (TnT4R).
Epitope	FMPNLVPPKI (5’ region of the cardiac gene exon 10). Cellular Localization: Mostly over the I bands in myofibrils.
Specificity	Recognizes the cardiac isoform of rabbit Troponin T (cTnT). Species Crossreactivity: canine, chicken, goldfish, guinea pig, human, mouse, porcine, rat and sparrow. Does not react with frog.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.