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You are here:Home » Antibodies » Abs to Cardiac Markers » Anti -TNNT2 (Troponin T, Cardiac Muscle, TnTc, Cardiac Muscle Troponin T, cTnT)

Anti -TNNT2 (Troponin T, Cardiac Muscle, TnTc, Cardiac Muscle Troponin T, cTnT)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
This antibody is specific for human cardiac troponin subunit T, a 31kD protein involved in transmitting the Ca2+-binding signals responsible for contraction of cardiac muscle. It reacts with subunit T of the troponin complex in human cells. It also cross-reacts with human skeletal troponin T (23.2% by ELISA).
Catalog #T8665-24D
ApplicationsSuitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:50 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Positive ControlCardiac muscle
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No2D31
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHighly purified human cardiac troponin T
SpecificityRecognizes human cardiac troponin T.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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