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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Trypsin Inhibitor, Soy Bean (HRP)

Anti -Trypsin Inhibitor, Soy Bean (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH
The soybean trypsin inhibitor was first crystallized by Kunitz in 1945 and is one of several such inhibitors found in soybeans. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly inhibited by soy inhibitor.
Catalog #T8685-05
ApplicationsSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:10,000-1:40,000
Western Blot: 1:5000-1:20,000
Immunohistochemistry: 1:200-1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
PurityPuri ed from monospeci c antiserum by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenTrypsin Inhibitor (Soy Bean).
SpecificityRecognizes Trypsin Inhibitor. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti peroxidase, anti rabbit serum, as well as purified and partially purified trypsin inhibitor (soy bean).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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