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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Trypsin Inhibitor

Anti -Trypsin Inhibitor

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B
Lima bean trypsin inhibitor acts upon both trypsin and chymotrypsin by forming equimolar complexes. Lima bean inhibitors may be chromatographically separated into as many as six variants. Jones et al., Biochem., 2, 66, (1963) characterized four of them. All have similar but not identical amino acid composition, contain six or seven disulfide bonds and lack methionine and tryptophan. Molecular weights vary between 8 kDa and 10 kDa
Catalog #T8685-11
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:4000-1:25,000. Assayed against 1ug of Trypsin Inhibitor in a standard sandwich ELISA using peroxidase conjugated affinity purified goat anti-rabbit IgG [H&L] and ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:500-1:3000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPuri ed by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenTrypsin Inhibitor [Lima Bean]
SpecificityRecognizes Trysoin inhibitor. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as puri ed and partially puri ed Trypsin Inhibitor [Lima Bean].
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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