Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E B

Lima bean trypsin inhibitor acts upon both trypsin and chymotrypsin by forming equimolar complexes. Lima bean inhibitors may be chromatographically separated into as many as six variants. Jones et al., Biochem., 2, 66, (1963) characterized four of them. All have similar but not identical amino acid composition, contain six or seven disulfide bonds and lack methionine and tryptophan. Molecular weights vary between 8 kDa and 10 kDa
Catalog #	T8685-11
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:4000-1:25,000. Assayed against 1ug of Trypsin Inhibitor in a standard sandwich ELISA using peroxidase conjugated affinity purified goat anti-rabbit IgG [H&L] and ABTS (A0030) as a substrate for 30 minutes at RT.
	Western Blot: 1:500-1:3000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~10mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity	Puri ed by delipidation, salt fractionation and ion exchange chromatography.
Immunogen	Trypsin Inhibitor [Lima Bean]
Specificity	Recognizes Trysoin inhibitor. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as puri ed and partially puri ed Trypsin Inhibitor [Lima Bean].
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.