| Catalog # | T8695 |
| Applications | Suitable for use in ELISA, Immunohistochemistry. Other applications not tested. |
| Specificity Using a conjugate Tryptamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds | |
| Compounds Cross-reactivity ratio(a) Tryptamine-G-BSA 1 Tryptamine-G-BSA (b) 1/32 5 Hydroxytryptamine-G-BSA 1/50 5 Methoxytryptamine-G-BSA 1/60 Tryptamine 1/2,047 5 Methoxytryptophan-G-BSA 1/5,763 Tryptophan-G-BSA 1/37,20 5 Hydroxytryptophan-G-BSA 1/50,000 |
| (a) | Tryptamine-G-BSA concentration/unconjugated or conjugated indolealkylamine concentration at half displacement. |
| (b) : Non-reduced conjugate |
| G = Glutaraldehyde, BSA = Bovine Serum Albumin |
| Recommended dilution The antiserum was tested using the free floating PAP technique on rat raphe nuclei. The anti-conjugated Tryptamine antibodies stains well between a 1/2,000-1/5,000. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2. No preservative added. |
| Purity | Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration. |
| Immunogen | Tryptamine-Glutaraldehyde-BSA |
| Specificity | Recognizes protein-conjugated Tryptamine. See additional crossreactivity data. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
