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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Tryptase, beta2 (TPSB2, MCPT6, Mast Cell Protease 6)

Anti -Tryptase, beta2 (TPSB2, MCPT6, Mast Cell Protease 6)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IP N
As major neutral proteases present in the mast cells, tryptases function as mediators in the pathogenesis of asthema and other allergic and inflammatory disorders. Tryptase, beta2 (TPSB2) consists of a signal peptide (residues 1 to 21), a pro peptide (residues 22 to 31) and a mature chain (residues 32 to 275). The amino acid sequence of mouse TPSB2 is 88%, 78%, 76%, and 73% identical to that of mouse, human (TPSB2 and TPSAB1), chimpanzee, and canine.
Catalog #T8700-15
ApplicationsSuitable for use in Immunoprecipitation, ELISA and Neutralization. Other applications not tested.
Recommended DilutionsImmunoprecipitation: 25ug/ml immunoprecipitates recombinant mouse Tryptase, beta 2 from conditioned cell culture medium.
Neutralization: Measured by its ability to neutralize Recombinant Mouse Mast Cell Protease­6/Mcpt6 (1ug/ml) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10uM). The Neutralization Dose (ND50) is typically 3ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H69
HostRat
SourceMouse
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography. Endotoxin: <0.1EU/ug (LAL)
ImmunogenRecombinant protein corresponding to aa32-276 from mouse Tryptase, beta2 expressed in NSO cells (P21845).
SpecificityRecognizes mouse Tryptase, beta2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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