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You are here:Home » Culture Media » Media-Ingredients » Tryptone (BSE-Free)

Tryptone (BSE-Free)

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Specifications

Tryptone is a specially formulated enzymatic digest of casein, similar to Peptone from gelatin. It is used to supply higher growth rates when used in molecular biology media preparations such as YPD Broth and Agar. Used as a nitrogen source in culture media for fastidious and non-fastidious organisms. Also used to differentially select bacteria based on carbohydrate intake since Tryptone is carbohydrate deficient.
Catalog #T8750
Typical Amino Acid Profile Ala 2.8%, Arg 3.2%, Asp 6.7%, Cys 0.3%, Glu 18%, Gly 1.8%, His 2.4%, Ile 4.7%, Leu 7.7%, Lys 7.0%, Met 2.5%, Phe 4.1%, Pro 9.1%, Ser 5.1%, Thr 3.9%, Trp 1.0%, Tyr 1.3%, Val 6.0%
Appearance Light tan, homogenous, free flowing powder
Solubility Light yellow to amber, clear, complete
pH (2%) 6.5-7.5
Ash 15%
Moisture 5%
Amino Nitrogen 4%
Total Nitrogen 10%
Undigested Proteins Negative
Reducing SugarsNegative
Proteases Trace positive to positive
Nitrites Negative
Tryptophan Trace Positive to positive
Growth Supporting PropertiesAgar Formulation, Broth Formulation: Growth Test
Standard Plate Count: 5000cfu/g
Molds/ Yeasts: 100CFU/g
Coliforms: <10CFU/g
E. coli: Negative
Salmonella: Negative/25g
Certificate of OriginThe raw animal product used in the manufacturing of Tryptone is derived from casein, a milk product derived from bovine, originating in New Zealand where there have been no reported cases of bovine spongiform encephalopathy (BSE). Tryptone is processed in Mexico. During processing of the product, a batch is heated to a minimum of 80°C for 1 hour including a minimum of 5 minutes at 105°C or greater and then dried at a minimum of 140°C. The enzyme used in processing is porcine-derived from the USA.
CAS Number91079-40-2
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Certificate of Origin
The raw animal product used in the manufacturing of Tryptone is derived from casein, a milk product derived from bovine, originating in Australia where there have been no reported cases of bovine spongiform encephalopathy (BSE). During processing of the product, a batch is heated to a minimum of 80°C for 1 hour including a minimum of 5 minutes. at 105°C or greater and then dried at a minimum of 140°C.
Alternate namesPancreatic digest of casein; Casein hydrolysate; Trypticase; Enzyme hydrolyzed casein; Casitone; Casein peptone; NZ Amine


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