Pancreatic digest of casein; Casein hydrolysate; Trypticase; Enzyme hydrolyzed casein; Casitone; Casein peptone; NZ Amine
Tryptone is a specially formulated enzymatic digest of casein, similar to Peptone from gelatin. It is used to supply higher growth rates when used in molecular biology media preparations such as YPD Broth and Agar. Used as a nitrogen source in culture media for fastidious and non-fastidious organisms. Also used to differentially select bacteria based on carbohydrate intake since Tryptone is carbohydrate deficient.
Typical Amino Acid Profile
Ala 2.8%, Arg 3.2%, Asp 6.7%, Cys 0.3%, Glu 18%, Gly 1.8%, His 2.4%, Ile 4.7%, Leu 7.7%, Lys 7.0%, Met 2.5%, Phe 4.1%, Pro 9.1%, Ser 5.1%, Thr 3.9%, Trp 1.0%, Tyr 1.3%, Val 6.0%
Appearance
Light tan, homogenous, free flowing powder
Solubility
Light yellow to amber, clear, complete
Undigested Proteins
Negative
Proteases
Trace positive to positive
Tryptophan
Trace Positive to positive
Growth Supporting Properties
Agar Formulation, Broth Formulation: Growth Test Standard Plate Count: ≤5000cfu/g Molds/ Yeasts: ≤100CFU/g Coliforms: <10CFU/g E. coli: Negative Salmonella: Negative/25g
Certificate of Origin
The raw animal product used in the manufacturing of Tryptone is derived from casein, a milk product derived from bovine, originating in New Zealand where there have been no reported cases of bovine spongiform encephalopathy (BSE). Tryptone is processed in Mexico. During processing of the product, a batch is heated to a minimum of 80°C for 1 hour including a minimum of 5 minutes at 105°C or greater and then dried at a minimum of 140°C. The enzyme used in processing is porcine-derived from the USA.
Source
Enzymatic digest of bovine casein
Form
Light tan, homogenous, free flowing powder
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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