| Tryptone is a specially formulated enzymatic digest of casein, similar to Peptone from gelatin. It is used to supply higher growth rates when used in molecular biology media preparations such as YPD Broth and Agar. Used as a nitrogen source in culture media for fastidious and non-fastidious organisms. Also used to differentially select bacteria based on carbohydrate intake since Tryptone is carbohydrate deficient. |
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| Catalog # | T8750 |
| Typical Amino Acid Profile | Ala 2.8%, Arg 3.2%, Asp 6.7%, Cys 0.3%, Glu 18%, Gly 1.8%, His 2.4%, Ile 4.7%, Leu 7.7%, Lys 7.0%, Met 2.5%, Phe 4.1%, Pro 9.1%, Ser 5.1%, Thr 3.9%, Trp 1.0%, Tyr 1.3%, Val 6.0% |
| Appearance | Light tan, homogenous, free flowing powder |
| Solubility | Light yellow to amber, clear, complete |
| pH (2%) | 6.5-7.5 |
| Ash | 15% |
| Moisture | 5% |
| Amino Nitrogen | 4% |
| Total Nitrogen | 10% |
| Undigested Proteins | Negative |
| Reducing Sugars | Negative |
| Proteases | Trace positive to positive |
| Nitrites | Negative |
| Tryptophan | Trace Positive to positive |
| Growth Supporting Properties | Agar Formulation, Broth Formulation: Growth Test |
| Standard Plate Count: 5000cfu/g |
| Molds/ Yeasts: 100CFU/g |
| Coliforms: <10CFU/g |
| E. coli: Negative |
| Salmonella: Negative/25g |
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| Certificate of Origin | The raw animal product used in the manufacturing of Tryptone is derived from casein, a milk product derived from bovine, originating in Australia where there have been no reported cases of bovine spongiform encephalopathy (BSE). During processing of the product, a batch is heated to a minimum of 80°C for 1 hour including a minimum of 5 minutes. at 105°C or greater and then dried at a minimum of 140°C. |
| CAS Number | 91079-40-2 |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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| Certificate of Origin | |
| The raw animal product used in the manufacturing of Tryptone is derived from casein, a milk product derived from bovine, originating in Australia where there have been no reported cases of bovine spongiform encephalopathy (BSE). During processing of the product, a batch is heated to a minimum of 80°C for 1 hour including a minimum of 5 minutes. at 105°C or greater and then dried at a minimum of 140°C. |
| Alternate names | Pancreatic digest of casein; Casein hydrolysate; Trypticase; Enzyme hydrolyzed casein; Casitone; Casein peptone; NZ Amine |
