| Catalog # | T9013 |
| Applications | |
| Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. |
| Recommended Dilutions | Immunohistochemistry: 1:1000-1:5000 |
| Optimal dilutions to be determined by the researcher. |
| Crossreactivity | Using a conjugate L-Tryptophan-(PC), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: |
| Compounds | Crossreactivity Ratio (a): |
| L-Tryptophan-G-(Pc) 1 |
| 5-Hydroxy-L-Tryptophan-G-(Pc) 1/100 |
| 5-Methoxy-L-Tryptophan-G-(Pc) 1/250 |
| L-Tryptophan-GA-(Pc) 1/450 |
| L-Tryptophan 1/3000 |
| Tryptamine-G-(Pc) 1/11,000 |
| 5-Methoxytryptamine-G-(Pc) 1/56,000 |
| 5-Hydroxytryptamine-G-(Pc) 1/220,000 |
| (a) | Tryptophan-G-(Pc) concentration/other conjugated indolealkylamine concentration at half displacement. |
| G=Glutaraldehyde; GA=Glutaric anhydride |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid. |
| Purity | Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration. |
| Immunogen | Synthetic L-Tryptophan conjugated to a protein carrier |
| Specificity | Recognizes conjugated L-Tryptophan. See additional crossreactivity data. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
