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You are here:Home » Antibodies » Abs to Biochemicals » Anti -Tryptophan

Anti -Tryptophan

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E IH RIA
Catalog #T9013-05
ApplicationsSuitable for use in Immunohistochemistry, ELISA and RIA.
Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:1000-1:2500 by PAP
Optimal dilution determined by the researcher.
Crossreactivity DataThe crossreactivities were determined using either ELISA or RIA techniques, at concentration/unconjugated or conjugated amino acid concentration at half displacement.
Compound Crossreactivity Tryptophan-G-PL 1 5-Hydroxytryptophan-G-PL 0.009 5-Methoxytryptophan-G-PL 0.004
Tryptophan=G=PL (non reduced) 0.002
Tryptophan 0.0003 Tryptamine-G-PL 0.0009 5-Methoxytryptamine-G-PL 0.00002 5-Hydroxytryptamine-G-PL 0.000004
The antisera was also tested for specificity using the free-floating PAP technique on rat raphe nuclei.
Abbreviations (G) Glutaraldehyde
(=) Non-reduced conjugate
(PL) Poly-lysine
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid with 0.05% sodium azide.
PuritySerum
ImmunogenTryptophan-glutaraldehyde-Poly-lysine
SpecificityRecognizes Tryptophan. See additional Crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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