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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E IH RIA

Catalog #	T9013-05
Applications	Suitable for use in Immunohistochemistry, ELISA and RIA.
	Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:1000-1:2500 by PAP
	Optimal dilution determined by the researcher.
Crossreactivity Data	The crossreactivities were determined using either ELISA or RIA techniques, at concentration/unconjugated or conjugated amino acid concentration at half displacement.
Compound Crossreactivity	Tryptophan-G-PL 1 5-Hydroxytryptophan-G-PL 0.009 5-Methoxytryptophan-G-PL 0.004
	Tryptophan=G=PL (non reduced) 0.002
	Tryptophan 0.0003 Tryptamine-G-PL 0.0009 5-Methoxytryptamine-G-PL 0.00002 5-Hydroxytryptamine-G-PL 0.000004
	The antisera was also tested for specificity using the free-floating PAP technique on rat raphe nuclei.
Abbreviations	(G) Glutaraldehyde
	(=) Non-reduced conjugate
	(PL) Poly-lysine
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	Not determined
Form	Supplied as a liquid with 0.05% sodium azide.
Purity	Serum
Immunogen	Tryptophan-glutaraldehyde-Poly-lysine
Specificity	Recognizes Tryptophan. See additional Crossreactivity data.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.