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T9125-01C Rat Anti-TSKS (Testis Specific Kinase Substrate, Testis-specific Serine Kinase Substrate, MGC134104, STK22 Substrate 1, Stk22s1, TSKS1)

Specifications
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Swiss Prot
Q8CBR6
Isotype
IgG2
Clone Number
11D28
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

SK, also known as Tsukushi and Leucine-rich repeat-containing protein 54, is a member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins, which are characterized by leucine-rich repeats (LRR) flanked by conserved cysteines. Mouse TSK is a secreted glycoprotein that has a predicted molecular weight of 36.5kD and contains 9 LLR. Proteoglycan modification of TSK has not been shown. Estrogen treatment in breast cancer cell lines up-regulates TSK, and so TSK is proposed to be involved in hormonally regulated extracellular matrix remodeling. Mouse TSK shares 96%, 85%, 51%, and 49% sequence identity with rat, human, chicken, and Xenopus TSK, respectively.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Direct ELISA: 0.5-1ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
Myeloma cells with B cells from Balb/c mice.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to mouse TSK expressed in NSO cells (Q8CBR6).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse TSK. Species sequence homology: Recombinant human TSK (25%).
Conjugates
Pricing
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