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You are here:Home » Antibodies » Antibodies-Tubulin » Anti -Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Anti -Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3,
Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)


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Clone Host Grade Applications
Monoclonal Mouse Purified E B IF
There are three classes of the ~50kD tubulin protein: alpha, beta and gamma. The alpha and beta tubulins form a heterodimer that polymerize into the cylindrical microtubule fibers. Both alpha and beta tubulin bind GTP; however, only beta tubulin hydrolyzes GTP to GDP. This hydrolysis is a process that is linked to tubulin polymerization and microtubule formation. The alpha tubulin isomer can be modified by addition of a C-terminal tyrosine residue. This modification may influence polymerization rates. The gamma tubulin isomer is localized to centrosomes which compose the heart of the microtubule organizing center from which microtubule fibers emanate.
Catalog #T9154-05
ApplicationsSuitable for use in ELISA, Western Blot, screening of expression libraries, identification of tubulin on fixed, frozen tissue sections and staining of microtubular structures in fixed cells by Immunofluorescence microscopy and by light microscopy using PAP complex. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Immunofluorescence: 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3G294
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified from ascites by sequential precipitation with caprylic acid and ammonium sulfate solution.
ImmunogenPeptide corresponding to the position alpha (65-79) of Tubulin.
SpecificityRecognizes alpha tubulin of various species. Recognizes a N-terminal structural domain of alpha tubulin. Reacts with peptides that correspond to position alpha (65-79) of Tubulin; band at 50kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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