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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Anti -Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3,
Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IP IH RIA
Catalog #T9154-05D
Fusion Partners Spleen cells from immunized LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
ApplicationsImmunohistochemistry (frozen)
ELISA
Immunoprecipitation
Western Blot
Radioimmunoassay
Optimal working dilutions to be determined by researcher.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H3087
HostRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenYeast tubulin.
SpecificityRecognizes the alpha subunit of tubulin, specifically binding to so called Tyr-Tubulin, which is produced by post translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some crossreactivity with other proteins in certain circumstances, including E. Coli rec A and oxidized actin. Species Crossreactivity: 3H3087 reacts with tubulin from yeast, avian and mammalian.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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