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Abs to Tubulin
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Anti -Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Anti -Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
E B IP IH RIA |
|
| Catalog # | T9154-05D | | Fusion Partners | Spleen cells from immunized LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line. | | Applications | Immunohistochemistry (frozen) | | ELISA | | Immunoprecipitation | | Western Blot | | Radioimmunoassay | | Optimal working dilutions to be determined by researcher. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 3H3087 | | Host | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Yeast tubulin. | | Specificity | Recognizes the alpha subunit of tubulin, specifically binding to so called Tyr-Tubulin, which is produced by post translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some crossreactivity with other proteins in certain circumstances, including E. Coli rec A and oxidized actin. Species Crossreactivity: 3H3087 reacts with tubulin from yeast, avian and mammalian. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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