Anti -Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3,
Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH IC FC IF |
|
| The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin | | | filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with a/b-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. g-tubulin is necessary to nucleate polymerization of tubulin subunits to form | | | microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome | | | alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). | | | Catalog # | T9154-05K | | Applications | Suitable for use in Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000 | | Immunohistochemistry (Paraffin): 1:50 | | Immunofluorescence: 1:50 | | Flow Cytometry: 1:25 | | Optimal dilution determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide corresponding to the sequence of human a-tubulin. | | Specificity | Recognizes endogenous levels of huamn total a-tubulin protein at 52kD., Does not cross-react with recombinant b-tubulin. Species Crossreactivity: mouse, rat, monkey, bovine and Drosophilia. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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