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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Anti -Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3,
Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IC
Catalog #T9154-07
ApplicationsSuitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 0.1-1ug/ml of this lot detected a-Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-a-Tubulin (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates a-Tubulin (~50kD).
Optimal dilutions to be determined by the researcher.
Included Positive Antigen ControlNon-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.593
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified human brain a-Tubulin. The epitope recognized by this antibody has been mapped to aa426-450 (AALEKDYEEVGVDSVEGEGEEEGEE).
SpecificityRecognizes a-Tubulin, Mr 50-60kD. Species Crossreactivity: Human, mouse, rat, bovine, porcine, guinea pig and avian.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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