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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin beta (Beta-tubulin, Tubulin beta Chain)

Anti -Tubulin beta (Beta-tubulin, Tubulin beta Chain)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IP IH IC IF
Microtubules are polymers of tubulin, a dimer of two 55kD subunits, designated alpha and beta. Within the microtubule lattice, alpha-beta heterodimers associate in a head-to-tail fashion, giving rise to microtubule polarity. Tubulin binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Distinct human beta-tubulin isotypes are encoded namely M40 and 5 beta, beta 2. The tubulin beta gene maps to human chromosome 6 at 6p21.33. It is expressed in human adrenal gland, colon, kidney, lung and pancreas.
Catalog #T9155-01V
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:50-1:100
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Positive ControlLung
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Numbern/a
Clone TypeMonoclonal
IsotypeIgG1
Clone No11E382
HostMouse
SourceChicken
ConcentrationAs reported
FormSupplied as a liquid, 0.05% sodium azide.
PurityPurified
ImmunogenNative chick brain microtubules. Cellular Localization: Cytoplasmic.
SpecificityRecognizes chicken Tubulin, beta. Species Crossreactivity: bovine, porcine, gerbil, guinea pig, human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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