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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, beta 3 (Tubulin beta III, Tubulin beta 3 Chain, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4, TUBB4)

Anti -Tubulin, beta 3 (Tubulin beta III, Tubulin beta 3 Chain, TUBB 3, TUBB3, Neuron
Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4, TUBB4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Serum B IH IF
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with alpha/beta tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. y-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Catalog #T9155-03D
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:100
Immunofluorescence (IF-F): 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10E53
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
PuritySerum
ImmunogenSynthetic peptide corresponding to the C-terminal of human beta 3-tubulin protein, conjugated to KLH.
SpecificityRecognizes endogenous levels of total human B3-Tubulin.
Species crossreactivityMouse, rat. Does not react with tubulin isoforms expressed in non-neuronal cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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