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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, beta 3 (Tubulin beta III, Tubulin beta-3 Chain, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, CFEOM3A, MC1R, Tubulin beta 4, TUBB 4, TUBB4)

Anti -Tubulin, beta 3 (Tubulin beta III, Tubulin beta-3 Chain, TUBB 3, TUBB3, Neuron
Specific beta III Tubulin, beta-4, CFEOM3A, MC1R, Tubulin beta 4, TUBB 4, TUBB4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IP IF
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin
filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with a/b-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. y-tubulin is necessary to nucleate polymerization of tubulin subunits to form
microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from ompetitive
microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Catalog #T9155-03M
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence (IF-F): 1:200
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C1086
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
PurityPurified
ImmunogenSynthetic peptide corresponding to the carboxy terminus of human B3-tubulin.
SpecificityRecognizes endogenous levels of total B3-tubulin. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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