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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)

Anti -Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain,
DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein,
TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IC IF
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000kD. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.
Catalog #T9155-07
ApplicationsSuitable for use in Western Blot, Immunoflourescence and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500 detected beta-tubulin on 10ug of A431 lysates.
Indirect Immunoflourescence: Allows staining of microtubuli showing a low background only.
Immunocytochemistry: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa Cell lysate, A431 Cell lysate, MCF7 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No2Q1126
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide.
PurityPurified
ImmunogenTubulin purified form polycephalum myxamoebae
SpecificityRecognizes beta-tubulin at ~50kD. Shows a strong crossreactivity to different species. Species Crossreactivity: Myxomycetes, yeast, fungi, plants, insects, nematodes, parasites, mammalian
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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