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You are here:Home » Antibodies » Antibodies-Tubulin » Anti -Tubulin, beta 3 (Tubulin beta III, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4)

Anti -Tubulin, beta 3 (Tubulin beta III, TUBB 3, TUBB3, Neuron Specific beta III Tubulin,
beta-4, MC1R, Tubulin beta 4, TUBB 4)


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Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B IP IH IC
Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. In adult tissues, the distribution of beta III is almost exclusively neuron-specific.
Catalog #T9155-09
ApplicationsSuitable for use in Western Blot, Immunohistochemistry (formalin fixed frozen or paraffin embedded tissue sections, Immunocytochemistry (fixed cells), Immunoprecipitation and ELISA. Other applications not tested.
Recommended DilutionImmunohistochemistry: High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.
Western Blot: Detects a single band at 50kD corresponding to Class III beta-tubulin.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No2Q1052
FormSupplied as a liquid in PBS, 10mM sodium azide, 1mg/ml BSA, before the addition of glycerol to 40%.
PurityPurified by DEAE ion exchange chromatography.
ImmunogenSynthetic peptide corresponding to aa 443-450 (ESESQGPK) of human class III beta-tubulin (KLH).
SpecificityRecognizes the C-terminus pof the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells. Species Crossreactivity: Bovine, human, monkey, mouse, porcine and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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