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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, beta 3 (Tubulin beta III, Tubulin beta 3 Chain, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4, TUBB4)

Anti -Tubulin, beta 3 (Tubulin beta III, Tubulin beta 3 Chain, TUBB 3, TUBB3, Neuron
Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4, TUBB4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IC
Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. In adult tissues, the distribution of beta III is almost exclusively neuron-specific.
Catalog #T9155-11
ApplicationsSuitable for use in Immunocytochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunocytochemistry: 2.5-5ug/ml showed positive immunostaining for neuronal beta3 tubulin in PC12 cells xed with 4% paraformaldehyde and permeabilized with Triton X-100.
Western Blot: 0.05-1ug/ml detected neuronal beta3 tubulin in RIPA lysates from PC12 cells. PC12 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with T9155-11 (0.05ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No4i66
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.01M sodium phosphate, pH 7.0, 0.01M EDTA, 0.15M sodium chloride.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa436-450, C-MYEDDEEESEAQGPK of neuronal speci c beta3 tubulin conjugated to KLH. Epitope: EAQGPK
SpecificityRecognizes human neuronal speci c beta3 tubulin, Mr 50kD. Species Crossreactivity: mouse, rat and bovine. Broad species crossreactivity predicted based on conservation of immunogen sequence.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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