Anti -Tubulin, beta (BSA & Azide Free) (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH IF |
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| Catalog # | T9155-12X | | Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits | alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. | | Applications | Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilution | Western Blot: 0.5-1ug/ml for 2 hours RT. | | Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Note: No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Ls174T and MAD109 cells, Skin or lung. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG3 | | Clone No | 3F389 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See T9155-12. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Purified tubulin. Cellular Localization: Cytoplasmic | | Specificity | Recognizes human Tubulin, beta at ~55kD. | | Species Crossreactivity | Bovine, rabbit, mouse, chicken, rat, frog, moth and wheat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Tubulin, beta (BSA & Azide Free) (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001) |
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