Anti -Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3,
Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Serum |
B IP IH IC |
|
| Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. | | | Catalog # | T9155-52A | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:50,000 | | Immunohistochemistry (paraffin): 1:250-500 | | Immunocytochemistry: 1:250-500 | | Immunoprecipitation: 1:50 | | Optimal dilutions to be determined by the researcher. | | Positive Control | HeLa cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9H538 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.18M sodium chloride, 0.25% sodium azide, 0.25mM EDTA, 25mM Citric Acid, 0.5% BSA, 40% glycerol. | | Purity | Serum | | Immunogen | Synthetic peptide corresponding to residues near the N-terminus of human alpha Tubulin. | | Specificity | Recognizes human Tubulin alpha. Species Crossreactivity: mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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