Anti -Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain,
DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein,
TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
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| Catalog # | T9155-75 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western BlotA nalysis: 0.1-1ug/ml detects beta-Tubulin in RIPA lysates from 3T3/A31, PC-12, Jurkat and A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-beta-Tubulin (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | Positive Antigen Control | A0001-50 Non-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. | | Optimal dilutions to be determined by the researcher. | | Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2T16 | | Host | Mouse | | Source | Bovine | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in TBS with 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Purified bovine brain tubulin. Epitope recognized by this antibody has been mapped to aa412-430. | | Specificity | Recognizes bovine beta-Tubulin isotypes I, II, III, IVa, and IVb of, Mr 50kD. No crossreactivity with alpha or gamma tubulin. | | Species Crossreactivity | Human, rat and mouse. Predicted to have broad species crossreactivity based on sequence homology. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001) |
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