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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)

Anti -Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain,
DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein,
TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Catalog #T9155-75
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern BlotA nalysis: 0.1-1ug/ml detects beta-Tubulin in RIPA lysates from 3T3/A31, PC-12, Jurkat and A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-beta-Tubulin (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Positive Antigen ControlA0001-50 Non-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Optimal dilutions to be determined by the researcher.
Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2T16
HostMouse
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in TBS with 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified bovine brain tubulin. Epitope recognized by this antibody has been mapped to aa412-430.
SpecificityRecognizes bovine beta-Tubulin isotypes I, II, III, IVa, and IVb of, Mr 50kD. No crossreactivity with alpha or gamma tubulin.
Species CrossreactivityHuman, rat and mouse. Predicted to have broad species crossreactivity based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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