Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, gamma (TUBG, Tubulin gamma 1 Chain, TUBG1, Tubulin gamma 2 Chain, TUBG2, Gamma 1 Tubulin, Gamma 2 Tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma Tubulin Complex Component 1, GCP 1, GCP-1, MGC131994, Xgam)

Anti -Tubulin, gamma (TUBG, Tubulin gamma 1 Chain, TUBG1, Tubulin gamma 2 Chain, TUBG2,
Gamma 1 Tubulin, Gamma 2 Tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma Tubulin Complex
Component 1, GCP 1, GCP-1, MGC131994, Xgam)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IC
Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.
The abundance of Gamma tubulin is less than 1% pf the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings. Gamma tubulin 1 and Gamma tubulin 2 are different isoforms that are 97% homologous.
Catalog #T9156-40H
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunocytochemistry: 1-2ug/ml. Cells must be fixed prior to staining. Fix cells for 10 min in either methanol at -20°C and for 6 min in acetone at -20°C, or in methanol alone for 10 min at -20°C, or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at RT.
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9F154
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 434-449, EYHAATRPDYISWGTQ, from the C-Terminus of the human gamma Tubulin (KLH).
SpecificityRecognizes human gamma tubulin expressed on stomach, kidney, liver, lung, muscle, ovary, heart, colon, thymus, brain. Species Crossreactivity: mouse, rat, chicken, bovine, porcine, and plants.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links