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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, gamma, CT (TUBG, Tubulin gamma 1 Chain, TUBG1, Tubulin gamma 2 Chain, TUBG2, Gamma 1 Tubulin, Gamma 2 Tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma Tubulin Complex Component 1, GCP 1, GCP-1, MGC131994, Xgam)

Anti -Tubulin, gamma, CT (TUBG, Tubulin gamma 1 Chain, TUBG1, Tubulin gamma 2 Chain,
TUBG2, Gamma 1 Tubulin, Gamma 2 Tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma Tubulin
Complex Component 1, GCP 1, GCP-1, MGC131994, Xgam)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Globular tubulin subunits comprise the microtubule building block, with a/b-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. As a critical part of the microtubule-organizing center (MTOC), the third member of the tubulin superfamily, y-tubulin, is required for microtubule nucleation as well as centrosome duplication and spindle assembly (1,2, reviewed in 3). y-tubulin forms complexes of two different sizes: y-tubulin small complex (y-TuSC) and the larger y-tubulin ring complex (y-TuRC). Each complex consists of a number of y-tubulin complex proteins (GCPs) with y-tubulin itself being considered GCP1. GCP2-6 all share sequence similarity in 5 different regions and it is thought that these areas could play a role in the proper folding of the proteins (4). y-TuSC is composed of two y-tubulin molecules as well as GCP2 and GCP3. y-TuRC is made up of a ring of multiple copies of y-TuSC in addition to GCP4, 5, and 6. Another protein, GCP-WD/NEDD1, which lacks sequence similarity with the other GCPs, associates with the y-TuRC. GCP-WD/NEDD1 has been shown to regulate localization of the y-TuSC to spindles and centrosomes (5-8). In mammals, phosphorylation of y-tubulin at Ser131 by SADB controls the activity of the y-TuRC. The hypothesis is that this phosphorylation stabilizes the protein in a conformation that stimulates centrosome amplification (9).
Catalog #T9156-40M
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues near the C-terminus of human TUBG.
SpecificityRecognizes endogenous levels of total human TUBG. Species Crossreactivity: Monkey, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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