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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, beta 3, NT (Tubulin beta III, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4)

Anti -Tubulin, beta 3, NT (Tubulin beta III, TUBB 3, TUBB3, Neuron Specific beta III
Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC
Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is dependent on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors (3).
Catalog #T9157-30C
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:20,000
Immunohistochemistry: 1:250-1:500
Flow Cytometry: 1:30
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E608
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Beta-Tubulin class III
SpecificityRecognizes human Beta-Tubulin class III, NT at ~55kD. Species crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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