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You are here:Home » Molecular Biology » MB-Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Canine (TNFa)

Tumor Necrosis Factor alpha, Recombinant, Canine (TNFa)


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Catalog #T9160-02C
SourceRecombinant protein expressed in Pichia pastoris
DescriptionCanine Tumor Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the canine inflammatory response.
Biological ActivityThis product has an ED50 of 10pg/ml, determined by measuring the cytolytic effect of recombinant canine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D.
Endotoxin Level<1.1.EU/ug
ApplicationsSuitable for use in In vitro applications and ELISA. Other applications not tested.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume and store at -20°C. Reconstituted product is stable for 12 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17.3kD
Purity>95% by SDS PAGE analysis.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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